臺北學研究主題資料庫

現在位置: 首頁 > 熱門推薦 > 熱門書目排行榜 > 詳細資料
  • 書籤
  • 建立標籤
  • 標籤連結
  • 推推
  • 詮釋資料
  • 影像
  • 目錄
系統識別碼 091YM000380002
資料類型 學位論文
主要題名 台灣北投地熱谷嗜熱菌之分離與鑑定
作者 楊姵羚
出版日期 2000
頁碼或冊數 65
中文摘要 本實驗室自台灣台北市北投地熱谷溫泉水及其沉積物中,分離出一株極端嗜酸熱菌─TA,其細胞寬度為0.5-2.0 μm,在液體培養基中,細胞各自分離,不群聚,具有不規則的類球體型態,不具鞭毛,無運動性,兼性需氧,且革蘭氏染色為陰性。可在60 - 95℃範圍內生長,最適生長溫度為85℃左右,pH值範圍從2.0-6.0,pH 3.0為其最適生長酸鹼值,在pH 1.5下不生長,pH 6.0下生長相當緩慢。有氧狀態下,所培養菌體呈乳白色,無氧狀態下呈赭紅色,且多呈現Ghost (類似紅血球的ghost,即透明薄膜包裹小顆粒) 型態。將Ghost狀態的菌體接種入新的培養液裡,依舊能重新培養出子代,故假設此Ghost狀態的菌體可能為其休眠態,甚或是透明薄膜內孢子。除此之外,在沒有S0而有O2存在的情況下,可在實驗室中以Allen’s medium加入有機養分0.1 % yeast extract及0.1 %蔗糖於高溫震盪培養下,可行異營性生長。此嗜酸熱菌之G + C莫耳百分比為31 %,綜合觀察其外顯特性及16S rDNA序列比對結果,均與Acidianus ambivalens (DSM 3772)、Acidianus brierleyi (DSM 1651)、Acidianus infernus (DSM 3191)及Metallosphaera sedula等菌株較為接近,因此,我們暫且將此菌株與Acidianus spp.歸類在同一屬,並將之命名為Acidianus sp. TA.
英文摘要 In this thesis, we have isolated a strain of hyperthermophilic archaeon─ TA from Geothermal Valley in Bei Tou district, Taipei city, located in Northern Taiwan. The cell width ranges between 0.5 and 2.0μm, depending on the culture condition. This hyperthermophile is found to be single cells in liquid culture. It is shaped like an irregular sphere, with no flagellum and no mobility. It is Gram-negative and grows facultatively in both anaerobic and aerobic conditions. This organism is extremely thermophilic with guanine-plus cytosine (G + C) ratio of 31 mol %. Its optimal growth temperatures is 85 degree C and its optimal pH of growth is 3.0. They still thrive in the temperature range of from 60℃ to 95℃ and pH range of from 2.0 to 6.0. Although they were isolated in hot spring, they still could grow in the presence of 0.1 to 4 % NaCl. There are no growth when they were placed below pH 1.5 or above pH 6.0. It could maintain its reproductive ability under 37 degree C slowly.
相關網址 連結...
出版者 國立陽明大學微生物暨免疫學研究所
出版地 臺北市
關鍵詞 臺北市;北投區;台灣北投地熱谷;嗜熱菌;鑑定
布農族的番丁的盛裝與旅行用裝

Google Map

Google Maps JavaScript API Example: LocalSearch Control

檢視較大的地圖